• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

STAT3 Recombinant antibody, PBS Only

STAT3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H1

Cat no : 80149-1-PBS

Synonyms

Acute phase response factor, APRF, FLJ20882, HIES, STAT3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STAT3 Recombinant antibody (80149-1-RR)

    WB analysis using 80149-1-RR (same clone as 80149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAT3 Recombinant antibody (80149-1-RR)

    WB analysis using 80149-1-RR (same clone as 80149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STAT3 Recombinant antibody (80149-1-RR)

    WB analysis of HeLa using 80149-1-RR (same clone as 80149-1-PBS)

    WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

Product Information

80149-1-PBS targets STAT3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight88 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.