STAR Recombinant antibody

STAR Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, pig, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

5M6

Cat no : 80751-1-RR

Synonyms

START domain-containing protein 1, STARD1, 5M6

---

Validation Data Gallery

• 

  WB analysis using 80751-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80751-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80751-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 80751-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80751-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 80751-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  Affinity and Kinetic Characterization of 80751-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

---

Tested Applications

• Positive WB detected in: human testis tissue, K-562 cells, pig adrenal gland tissue, human adrenal gland tissue, rat testis tissue, rat adrenal gland tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

80751-1-RR targets STAR in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

• Tested Reactivity: Human, rat, pig, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STAR fusion protein Ag2862
• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2918910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.

Publications

Species	Application	Title
human,mouse	WB,IF	Cell Commun Signal; GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement; Authors - Yuwei Duan
mouse	WB	Ecotoxicol Environ Saf; 1-Nitropyrene disrupts testicular steroidogenesis via oxidative stress-evoked PERK-eIF2α pathway; Authors - Xiu-Liang Li