STAR Recombinant antibody, PBS Only

STAR Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5M6

Cat no : 80751-1-PBS

Synonyms

START domain-containing protein 1, STARD1, 5M6

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Validation Data Gallery

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  WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80751-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

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Product Information

80751-1-PBS targets STAR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

• Tested Reactivity: Human, rat, pig, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STAR fusion protein Ag2862
• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.