STAM Polyclonal antibody

• Featured Product
• KD/KO Validated

STAM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 12434-1-AP

Synonyms

STAM-1, STAM1, STAM 1

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Validation Data Gallery

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  WB analysis using 12434-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Multi-cells using 12434-1-AP

  WB result of 12434-1-aP (STAM antibody).

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  WB analysis of mouse brain using 12434-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12434-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 12434-1-AP

  IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12434-1-AP (STAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Multi-cells, mouse brain tissue, Y79 cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells, SKOV-3 cells, C2C12 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Product Information

12434-1-AP targets STAM in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAM fusion protein Ag3112
• Full Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC030586
• Gene Symbol: STAM
• Gene ID (NCBI): 8027
• RRID: AB_2199965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner
human	WB,IF	Cell Rep; TIM-1 Ubiquitination Mediates Dengue Virus Entry.; Authors - Ophélie Dejarnac ; KD Validated
		Development; Identification of STAM1 as a novel effector of ventral projection of spinal motor neurons.; Authors - Heejin Nam
human	IF,WB	PLoS Pathog; A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor.; Authors - Charlotte A Stoneham