Validation Data Gallery
|Positive WB detected in||HEK-293T cells, Multi-cells, mouse brain tissue, Y79 cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells, SKOV-3 cells, C2C12 cells|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human testis tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12434-1-AP targets STAM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STAM fusion protein Ag3112|
|Full Name||signal transducing adaptor molecule (SH3 domain and ITAM motif) 1|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC030586|
|Gene ID (NCBI)||8027|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)
|Product Specific Protocols|
|WB protocol for STAM antibody 12434-1-AP||Download protocol|
|IHC protocol for STAM antibody 12434-1-AP||Download protocol|
|IF protocol for STAM antibody 12434-1-AP||Download protocol|
|IP protocol for STAM antibody 12434-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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