ST2 Monoclonal antibody, PBS Only

ST2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7A2A7

Cat no : 60112-1-PBS

Synonyms

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1

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Validation Data Gallery

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  WB analysis using 60112-1-Ig (same clone as 60112-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60112-1-Ig (same clone as 60112-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60112-1-Ig (same clone as 60112-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 60112-1-Ig (same clone as 60112-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60112-1-Ig (same clone as 60112-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60112-1-Ig (same clone as 60112-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60112-1-Ig (same clone as 60112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60112-1-Ig (same clone as 60112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60112-1-PBS targets ST2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ST2 fusion protein Ag2555
• Full Name: interleukin 1 receptor-like 1
• Calculated Molecular Weight: 63 kDa, 37 kDa, 30 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC030975
• Gene Symbol: IL1RL1
• Gene ID (NCBI): 9173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)