IL-1RL1/ST2 Monoclonal antibody
IL-1RL1/ST2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
7A2A7
Cat no : 60112-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 3 publications below |
IF | See 8 publications below |
Product Information
60112-1-Ig targets IL-1RL1/ST2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-1RL1/ST2 fusion protein Ag2555 |
Full Name | interleukin 1 receptor-like 1 |
Calculated Molecular Weight | 63 kDa, 37 kDa, 30 kDa |
Observed Molecular Weight | 63 kDa |
GenBank Accession Number | BC030975 |
Gene Symbol | IL1RL1 |
Gene ID (NCBI) | 9173 |
RRID | AB_2264785 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)
Protocols
Product Specific Protocols | |
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WB protocol for IL-1RL1/ST2 antibody 60112-1-Ig | Download protocol |
IHC protocol for IL-1RL1/ST2 antibody 60112-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Immunity Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis. | ||
Neurology Association of Soluble ST2 With Functional Outcome, Perihematomal Edema, and Immune Response After Intraparenchymal Hemorrhage | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Sci Rep Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model. | ||
Cancer Biomark Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model | ||