IL-1RL1/ST2 Monoclonal antibody

IL-1RL1/ST2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

7A2A7

Cat no : 60112-1-Ig

Synonyms

IL1RL1, IL-1RL1, FIT 1, EC:3.2.2.6, DER4



Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60112-1-Ig targets IL-1RL1/ST2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1RL1/ST2 fusion protein Ag2555
Full Name interleukin 1 receptor-like 1
Calculated Molecular Weight 63 kDa, 37 kDa, 30 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC030975
Gene Symbol IL1RL1
Gene ID (NCBI) 9173
RRIDAB_2264785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Protocols

Product Specific Protocols
WB protocol for IL-1RL1/ST2 antibody 60112-1-IgDownload protocol
IHC protocol for IL-1RL1/ST2 antibody 60112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunity

Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.

Authors - Zuojia Chen
humanIF

Neurology

Association of Soluble ST2 With Functional Outcome, Perihematomal Edema, and Immune Response After Intraparenchymal Hemorrhage

Authors - Matthew B Bevers
humanWB,IHC

Sci Rep

Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.

Authors - Elena Vianello
humanIF

Sci Rep

Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.

Authors - Shuhei Kobayashi
mouseIF

Cancer Biomark

Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model

Authors - Shan Wang
humanIF, WB

Inflamm Res

Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.

Authors - Jingxi Zhang