SSSCA1 Monoclonal antibody, PBS Only

SSSCA1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-PBS

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

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Validation Data Gallery

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  WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68423-1-PBS targets SSSCA1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SSSCA1 fusion protein Ag16783
• Full Name: Sjogren syndrome/scleroderma autoantigen 1
• Calculated Molecular Weight: 199 aa, 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC014791
• Gene Symbol: SSSCA1
• Gene ID (NCBI): 10534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.