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  • Western Blot (WB) analysis of various lysates using SSSCA1 Monoclonal antibody (68423-1-Ig)

    WB analysis using 68423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SSSCA1 Monoclonal antibody (68423-1-Ig)

    IF Staining of HeLa using 68423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68423-1-Ig
Clone No.1D2E8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68423-1-Ig targets SSSCA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag16783 Predict reactive species
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC014791
Gene Symbol SSSCA1
Gene ID (NCBI) 10534
RRIDAB_3085138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

Protocols

Product Specific Protocols
WB protocol for SSSCA1 antibody 68423-1-IgDownload protocol
IF protocol for SSSCA1 antibody 68423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols