SRY Monoclonal antibody, PBS Only

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  WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 67349-1-Ig (same clone as 67349-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

SRY Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67349-1-PBS

Clone No.2E9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Testis-determining factor, Sex-determining region Y protein, sex determining region Y, 2E9A4

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Product Information

67349-1-PBS targets SRY in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRY fusion protein Ag12610
• Full Name: sex determining region Y
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC074924
• Gene Symbol: SRY
• Gene ID (NCBI): 6736
• RRID: AB_2882607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05066
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.