SREBF2 Recombinant antibody, PBS Only (Detector)

SREBF2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240451A12

Cat no : 83403-1-PBS

Synonyms

SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2

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Validation Data Gallery

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  IF Staining of HepG2 using 83403-1-RR (same clone as 83403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83403-1-RR (same clone as 83403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00408-2

  Cytometric bead array standard curve of MP00408-2, SREBF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83403-2-PBS. Detection antibody: 83403-1-PBS. Standard: Ag28205. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00408-3

  Cytometric bead array standard curve of MP00408-3, SREBF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83403-4-PBS. Detection antibody: 83403-1-PBS. Standard: Ag28205. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83403-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

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Product Information

83403-1-PBS targets SREBF2 as part of a matched antibody pair:

MP00408-2: 83403-2-PBS capture and 83403-1-PBS detection (validated in Cytometric bead array)

MP00408-3: 83403-4-PBS capture and 83403-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SREBF2 fusion protein Ag28205
• Full Name: sterol regulatory element binding transcription factor 2
• Calculated Molecular Weight: 124 kDa
• GenBank Accession Number: BC056158
• Gene Symbol: SREBF2
• Gene ID (NCBI): 6721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.