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SREBF2 Recombinant antibody

SREBF2 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240451A12

Cat no : 83403-1-RR

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF2 Recombinant antibody (83403-1-RR)

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF2 Recombinant antibody (83403-1-RR)

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83403-1-RR is SREBF2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SREBF2 fusion protein Ag28205
Full Name sterol regulatory element binding transcription factor 2
Calculated Molecular Weight 124 kDa
GenBank Accession NumberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.

Protocols

Product Specific Protocols
IF protocol for SREBF2 antibody 83403-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols