SRD5A1 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

SRD5A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5D8

Cat no : 66329-1-PBS

Synonyms

Steroid 5 alpha reductase 1, SR type 1, S5AR 1, EC:1.3.1.22, 3-oxo-5-alpha-steroid 4-dehydrogenase 1

---

Validation Data Gallery

• 

  WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50930-1

  Cytometric bead array standard curve of MP50930-1, SRD5A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66329-1-PBS. Detection antibody: 66329-2-PBS. Standard:Ag23351. Range: 0.781-100 ng/mL.

---

Product Information

66329-1-PBS targets SRD5A1 as part of a matched antibody pair:

MP50930-1: 66329-1-PBS capture and 66329-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRD5A1 fusion protein Ag23351
• Full Name: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC007033
• Gene Symbol: SRD5A1
• Gene ID (NCBI): 6715
• RRID: AB_2881710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.