FDFT1 Recombinant antibody, PBS Only (Capture/Detector)

FDFT1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230362F3

Cat no : 83020-2-PBS

Synonyms

Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00097-1

  Sandwich ELISA standard curve of MP00097-1, Human FDFT1 Recombinant Matched Antibody Pair - PBS only. 83020-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3743. 83020-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP00097-1

  Serum of eight individual healthy human donors was measured. The FDFT1 concentration of detected samples was determined to be 1,817 pg/mL with a range of 368 - 5,103 pg/mL.

• 

  Sample test of MP00097-1

  The mean FDFT1 concentration was determined to be 8.7 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of HepG2 using 83020-2-RR (same clone as 83020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-2-RR, Clone: 230362F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83020-2-RR (same clone as 83020-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug FDFT1 Recombinant antibody (83020-2-RR, Clone:230362F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00097-1

  Cytometric bead array standard curve of MP00097-1, SQS/FDFT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83020-2-PBS. Detection antibody: 83020-4-PBS. Standard: Ag3743. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00097-3

  Cytometric bead array standard curve of MP00097-3, SQS/FDFT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83020-3-PBS. Detection antibody: 83020-2-PBS. Standard: Ag3743. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83020-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83020-2-RR against Human FDFT1 were performed. The affinity constant is 0.107 nM.

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Product Information

83020-2-PBS targets FDFT1 as part of a matched antibody pair:

MP00097-1: 83020-2-PBS capture and 83020-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00097-3: 83020-3-PBS capture and 83020-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FDFT1 fusion protein Ag3743
• Full Name: farnesyl-diphosphate farnesyltransferase 1
• Calculated Molecular Weight: 417 aa, 48 kDa
• GenBank Accession Number: BC029641
• Gene Symbol: FDFT1
• Gene ID (NCBI): 2222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.