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FDFT1 Recombinant antibody

FDFT1 Recombinant Antibody for IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230362F3

Cat no : 83020-2-RR

Synonyms

DGPT, ERG9, FDFT1, FPP:FPP farnesyltransferase, SQS, Squalene synthase, SS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FDFT1 Recombinant antibody (83020-2-RR)

    IF Staining of HepG2 using 83020-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-2-RR, Clone: 230362F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U2OS cells using FDFT1 Recombinant antibody (83020-2-RR)

    FC experiment of U2OS using 83020-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug FDFT1 Recombinant antibody (83020-2-RR, Clone:230362F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:100-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83020-2-RR targets FDFT1 in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDFT1 fusion protein Ag3743
Full Name farnesyl-diphosphate farnesyltransferase 1
Calculated Molecular Weight 417 aa, 48 kDa
GenBank Accession NumberBC029641
Gene Symbol FDFT1
Gene ID (NCBI) 2222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Protocols

Product Specific Protocols
IF protocol for FDFT1 antibody 83020-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols