FDFT1 Recombinant antibody

FDFT1 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230362C10

Cat no : 83020-1-RR

Synonyms

DGPT, ERG9, FDFT1, FPP:FPP farnesyltransferase, SQS, Squalene synthase, SS

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Validation Data Gallery

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  WB analysis using 83020-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 83020-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: L02 cells, T-47D cells, SH-SY5Y cells, HepG2 cells, mouse testis tissue
• Positive FC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83020-1-RR targets FDFT1 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FDFT1 fusion protein Ag3743
• Full Name: farnesyl-diphosphate farnesyltransferase 1
• Calculated Molecular Weight: 417 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC029641
• Gene Symbol: FDFT1
• Gene ID (NCBI): 2222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).