SQRDL Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SQRDL Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4E3

Cat no : 68459-1-PBS

Synonyms

Sulfide dehydrogenase-like, SQOR, EC:1.8.5.8, CGI-44, CGI 44

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Validation Data Gallery

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  WB analysis of A549 using 68459-1-Ig (same clone as 68459-1-PBS)

  WB result of SQRDL antibody (68459-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SQRDL transfected A549 cells. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  WB analysis using 68459-1-Ig (same clone as 68459-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68459-1-Ig (SQRDL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68459-1-Ig (same clone as 68459-1-PBS)

  IP result of anti-SQRDL (IP:68459-1-Ig, 4ug; Detection:68459-1-Ig 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 68459-1-Ig (same clone as 68459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68459-1-Ig (SQRDL antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68459-1-Ig (same clone as 68459-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQRDL antibody (68459-1-Ig, Clone: 1B4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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Product Information

68459-1-PBS targets SQRDL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SQRDL fusion protein Ag10762
• Full Name: sulfide quinone reductase-like (yeast)
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC016836
• Gene Symbol: SQRDL
• Gene ID (NCBI): 58472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.