SPTBN1 Monoclonal antibody, PBS Only

SPTBN1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1D1

Cat no : 67978-1-PBS

Synonyms

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

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Validation Data Gallery

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  WB analysis using 67978-1-Ig (same clone as 67978-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67978-1-Ig (SPTBN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 67978-1-Ig (same clone as 67978-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67978-1-Ig (SPTBN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67978-1-Ig (same clone as 67978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67978-1-Ig (SPTBN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

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Product Information

67978-1-PBS targets SPTBN1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPTBN1 fusion protein Ag31713
• Full Name: spectrin, beta, non-erythrocytic 1
• Calculated Molecular Weight: 2364 aa, 275 kDa
• Observed Molecular Weight: 250-275 kDa
• GenBank Accession Number: BC137282
• Gene Symbol: SPTBN1
• Gene ID (NCBI): 6711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.