SPTBN1 Monoclonal antibody
SPTBN1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, rabbit, pig and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1E1D1
Cat no : 67978-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse cerebellum tissue, HepG2 cells, Jurkat cells, pig brain, rabbit brain, rat brain, mouse brain, rat cerebellum |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
67978-1-Ig targets SPTBN1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SPTBN1 fusion protein Ag31713 |
| Full Name | spectrin, beta, non-erythrocytic 1 |
| Calculated Molecular Weight | 2364 aa, 275 kDa |
| Observed Molecular Weight | 250-275 kDa |
| GenBank Accession Number | BC137282 |
| Gene Symbol | SPTBN1 |
| Gene ID (NCBI) | 6711 |
| RRID | AB_2918727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTBN1 antibody 67978-1-Ig | Download protocol |
| IHC protocol for SPTBN1 antibody 67978-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Cancer Clinical significance of nonerythrocytic spectrin Beta 1 (SPTBN1) in human kidney renal clear cell carcinoma and uveal melanoma: a study based on Pan-Cancer Analysis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marina (Verified Customer) (03-15-2024) | Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:5000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The bands appear at the expected size of the target protein.
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