SPTA1 Polyclonal antibody

SPTA1 Polyclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55227-1-AP

Synonyms

EL2, Alpha I spectrin

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Validation Data Gallery

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  WB analysis using 55227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55227-1-AP (SPTA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse lung using 55227-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using SPTA1 antibody (55227-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 55227-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug SPTA1 Polyclonal antibody (55227-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat red blood cells, human blood
• Positive IF-P detected in: mouse lung tissue
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55227-1-AP targets SPTA1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
• Calculated Molecular Weight: 280 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: NM_003126
• Gene Symbol: SPTA1
• Gene ID (NCBI): 6708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha 1 Spectrin also known as SPTA1, EL2, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTA1 result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3(PMID: 1541680, 8941647).