SPRR3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11742-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11742-1-AP (same clone as 11742-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

  IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

  Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

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Product Information

11742-1-PBS targets SPRR3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRR3 fusion protein Ag2337
• Full Name: small proline-rich protein 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC017802
• Gene Symbol: SPRR3
• Gene ID (NCBI): 6707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.