SPRR3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11742-1-AP

Synonyms

SPRC, Small proline-rich protein 3

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Validation Data Gallery

• 

  WB analysis of HeLa using 11742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11742-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 11742-1-AP

  IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug.

• 

  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 11742-1-AP

  Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11742-1-AP targets SPRR3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRR3 fusion protein Ag2337
• Full Name: small proline-rich protein 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC017802
• Gene Symbol: SPRR3
• Gene ID (NCBI): 6707
• RRID: AB_2195272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.

Publications

Species	Application	Title
human,mouse,rat	WB,IP	FASEB J; Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.; Authors - Sarika Saraswati ; KO Validated
human	WB,IHC	Mol Med; Upregulation of SPRR3 promotes colorectal tumorigenesis.; Authors - Cho Dong-Hyung DH ; KD Validated
human	WB,IHC	Breast Cancer Res Treat; Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.; Authors - Kim Jin Cheon JC
human	WB	Arch Biochem Biophys; Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.; Authors - Miki Kuribayashi
human	WB	Onco Targets Ther; Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer.; Authors - Qin Li
human	WB	bioRxiv; Discovery of novel microRNA regulators of ribosome biogenesis; Authors - Carson J Bryant ; KD Validated

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