Filter: AllWBIHC

WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)
Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 66618-2-Ig (same clone as 66618-2-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of Raji using 66618-2-Ig (same clone as 66618-2-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of rat spleen using 66618-2-Ig (same clone as 66618-2-PBS)
rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

View 3D epitope

SPI1 Monoclonal antibody, PBS Only

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66618-2-PBS

Clone No.2H3D3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SFPI1, PU.1, OF, 31 kDa-transforming protein, 31 kDa transforming protein

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: --

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66618-2-PBS targets SPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated Molecular Weight	31 kDa
Observed Molecular Weight	32-38 kDa
GenBank Accession Number	NM_003120
Gene Symbol	SPI1
Gene ID (NCBI)	6688
ENSEMBL Gene ID	ENSG00000066336
RRID	AB_2881978
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17947
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.