SPI1 Monoclonal antibody

SPI1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2H3D3

Cat no : 66618-2-Ig

Synonyms

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

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Validation Data Gallery

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  WB analysis of Daudi using 66618-2-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66618-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66618-2-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66618-2-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, RAW 264.7 cells, rat spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

66618-2-Ig targets SPI1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: NM_003120
• Gene Symbol: SPI1
• Gene ID (NCBI): 6688
• RRID: AB_2881978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Publications

Species	Application	Title
human	IHC,WB	Discov Oncol; SPI1 is a prognostic biomarker of immune infiltration and immunotherapy efficacy in clear cell renal cell carcinoma; Authors - Huayi Feng
human	WB	Cancers (Basel); ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors; Authors - Qiyi Yi
human	WB,IF,CoIP	Exp Cell Res; IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis; Authors - Yilin Chen
mouse	IHC	Biomedicines; Exploring the Role of Neutrophil-Related Genes in Osteosarcoma via an Integrative Analysis of Single-Cell and Bulk Transcriptome; Authors - Jing Lu