SPARC Recombinant antibody, PBS Only (Capture)

SPARC Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241952A12

Cat no : 84621-1-PBS

Synonyms

ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP01430-2

  Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

• 

  Sample test of MP01430-2

  Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

• 

  Sample test of MP01430-2

  Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

• 

  Sample test of MP01430-2

  MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

• 

  Cytometric bead array standard curve of MP01430-1

  Cytometric bead array standard curve of MP01430-1, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-2-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

• 

  Cytometric bead array standard curve of MP01430-2

  Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

---

Product Information

84621-1-PBS targets SPARC as part of a matched antibody pair:

MP01430-1: 84621-1-PBS capture and 84621-2-PBS detection (validated in Cytometric bead array)

MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Calculated Molecular Weight: 35kDa
• GenBank Accession Number: NM_003118.4
• Gene Symbol: SPARC
• Gene ID (NCBI): 6678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.