SPAG11A Polyclonal antibody, PBS Only

SPAG11A Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16770-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 16770-1-AP (same clone as 16770-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

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Product Information

16770-1-PBS targets SPAG11A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG11A fusion protein Ag9846
• Full Name: sperm associated antigen 11A
• Calculated Molecular Weight: 123 aa, 14 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC058833
• Gene Symbol: SPAG11A
• Gene ID (NCBI): 653423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.