SPAG11A Polyclonal antibody

SPAG11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16770-1-AP

Synonyms

EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A

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Validation Data Gallery

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  WB analysis of human testis using 16770-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16770-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, human brain tissue
• Positive IHC detected in: human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

16770-1-AP targets SPAG11A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG11A fusion protein Ag9846
• Full Name: sperm associated antigen 11A
• Calculated Molecular Weight: 123 aa, 14 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC058833
• Gene Symbol: SPAG11A
• Gene ID (NCBI): 653423
• RRID: AB_2286582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.

Publications

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.; Authors - Pujianto Dwi A DA