SOX9 Monoclonal antibody

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  WB analysis using 67439-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human placenta using 67439-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 67439-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67439-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 67439-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOX9 (67439-1-Ig, Clone:1H9A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 67439-1-Ig

Clone No.1H9A6

Publications(79)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (4)

Applications

WB, FC (Intra), ELISA

Synonyms

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

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Tested Applications

• Positive WB detected in: HT-29 cells, Caco-2 cells, human placenta tissue, NCCIT cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 59 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Product Information

67439-1-Ig targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX9 fusion protein Ag28620
• Full Name: SRY (sex determining region Y)-box 9
• Calculated Molecular Weight: 509 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC007951
• Gene Symbol: SOX9
• Gene ID (NCBI): 6662
• RRID: AB_2882675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P48436
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,rat		Bioact Mater; Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair; Authors - Changjian Lin
human	WB	Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
human,mouse	WB,IF	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
rat	WB	J Nanobiotechnology; Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy; Authors - Linjun Yang
mouse	WB	Free Radic Biol Med; Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes; Authors - Wenchao Wang