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SOX9 Monoclonal antibody

SOX9 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (5)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9A6

Cat no : 67439-1-Ig

Synonyms

CMD1, CMPD1, SOX9, SRA1, Transcription factor SOX 9



Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells, human placenta tissue, NCCIT cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67439-1-Ig targets SOX9 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat, rabbit, pig, canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX9 fusion protein Ag28620
Full Name SRY (sex determining region Y)-box 9
Calculated Molecular Weight 509 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC007951
Gene Symbol SOX9
Gene ID (NCBI) 6662
RRIDAB_2882675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for SOX9 antibody 67439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human,mouseWB,IF

Theranostics

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai
ratWB

J Nanobiotechnology

Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy

Authors - Linjun Yang
mouseWB

Free Radic Biol Med

Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes

Authors - Wenchao Wang
humanWB

EBioMedicine

Decreased miR-214-3p activates NF-κB pathway and aggravates osteoarthritis progression.

Authors - Yumei Cao