SOX9 Monoclonal antibody

SOX9 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (4)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

1H9A6

Cat no : 67439-1-Ig

Synonyms

CMD1, CMPD1, SOX9, SRA1, Transcription factor SOX 9

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Validation Data Gallery

• 

  WB analysis of human placenta using 67439-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67439-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67439-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, Caco-2 cells, HT-29 cells, NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

67439-1-Ig targets SOX9 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, rat, canine, rabbits
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX9 fusion protein Ag28620
• Full Name: SRY (sex determining region Y)-box 9
• Calculated molecular weight: 509 aa, 56 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: BC007951
• Gene symbol: SOX9
• Gene ID (NCBI): 6662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determiningregion Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Med Sci Monit; Long Non-Coding RNA THOR Enhances the Stem Cell-Like Traits of Triple-Negative Breast Cancer Cells Through Activating β-Catenin Signaling.; Authors - Binbin Wang
rat	WB	Front Biosci (Landmark Ed); The biological effect of cobalt chloride mimetic-hypoxia on nucleus pulposus cells and the comparability with physical hypoxia in vitro.; Authors - Xiao-Xin Gao
rabbits	WB	J Orthop Surg Res; Hyaluronic acid hydrogel encapsulated BMP-14-modified ADSCs accelerate cartilage defect repair in rabbits.; Authors - Hao Liu
human	WB	J Orthop Surg Res; LncRNA NONHSAT030515 promotes the chondrogenic differentiation of human adipose-derived stem cells via regulating the miR-490-5p/BMPR2 axis.; Authors - Qinqin Yang
rat	WB	Evid Based Complement Alternat Med; Electroacupuncture Upregulates HIF-1α and SOX9 Expression in Knee Osteoarthritis.; Authors - Jie Tong
human	WB	Virus Genes; SOX9 negatively regulates the RLR antiviral signaling by targeting MAVS.; Authors - Xue Jiang ; KO Validated