SOD1 Recombinant antibody

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  WB analysis using 83857-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83857-6-RR (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83857-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83857-6-RR against Human SOD1 were performed. The affinity constant is 13.0 nM.

SOD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83857-6-RR

Clone No.240888B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83857-6-RR targets SOD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: superoxide dismutase 1, soluble
• Calculated Molecular Weight: 16kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: NM_000454.5
• Gene Symbol: SOD1
• Gene ID (NCBI): 6647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00441
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.