SOCS3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SOCS3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2B12

Cat no : 66797-1-PBS

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3

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Validation Data Gallery

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  WB analysis using 66797-1-Ig (same clone as 66797-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66797-1-Ig (same clone as 66797-1-PBS)

  WB result of SOCS3 antibody (66797-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66797-1-Ig (same clone as 66797-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SOCS3 antibody (66797-1-Ig, Clone: 1G2B12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66797-1-PBS targets SOCS3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOCS3 fusion protein Ag5386
• Full Name: suppressor of cytokine signaling 3
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC060858
• Gene Symbol: SOCS3
• Gene ID (NCBI): 9021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).