CoraLite® Plus 488-conjugated SNX9 Monoclonal antibody
SNX9 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B11C9
Cat no : CL488-68255
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-68255 targets SNX9 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | SNX9 fusion protein Ag8446 |
Full Name | sorting nexin 9 |
Calculated Molecular Weight | 595 aa, 67 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC005022 |
Gene Symbol | SNX9 |
Gene ID (NCBI) | 51429 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 SNX9 antibody CL488-68255 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |