CoraLite®594-conjugated SNX5 Monoclonal antibody

SNX5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F8A4

Cat no : CL594-67665

Synonyms

Sorting nexin-5, 2F8A4

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67665

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated SNX5 antibody (CL594-67665, Clone: 2F8A4 ) at dilution of 1:100.

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  FC experiment of HeLa using CL594-67665

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SNX5 (CL594-67665, Clone:2F8A4) (red), or 0.4 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67665 targets SNX5 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNX5 fusion protein Ag12330
• Full Name: sorting nexin 5
• Calculated Molecular Weight: 404 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC093623
• Gene Symbol: SNX5
• Gene ID (NCBI): 27131
• RRID: AB_2920168
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).