SNX18 Recombinant antibody
SNX18 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241537D11
Cat no : 84215-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse brain tissue, MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 84215-4-RR is SNX18 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SNX18 fusion protein Ag16645 |
Full Name | sorting nexin 18 |
Calculated Molecular Weight | 628 aa, 69 kDa |
Observed Molecular Weight | 68-70 kDa |
GenBank Accession Number | BC060791 |
Gene Symbol | SNX18 |
Gene ID (NCBI) | 112574 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SNX18 antibody 84215-4-RR | Download protocol |
IF protocol for SNX18 antibody 84215-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |