SNX18 Recombinant antibody, PBS Only

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  WB analysis using 84215-4-RR (same clone as 84215-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 84215-4-RR (same clone as 84215-4-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84215-4-RR (same clone as 84215-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-4-RR, Clone: 241537D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84215-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84215-4-RR against Human SNX18 were performed. The affinity constant is 0.378 nM.

SNX18 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84215-4-PBS

Clone No.241537D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11

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Product Information

84215-4-PBS targets SNX18 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNX18 fusion protein Ag16645
• Full Name: sorting nexin 18
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC060791
• Gene Symbol: SNX18
• Gene ID (NCBI): 112574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96RF0
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).