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SNX18 Recombinant antibody, PBS Only

SNX18 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241537D11

Cat no : 84215-4-PBS

Synonyms

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX18 Recombinant antibody (84215-4-RR)

    WB analysis using 84215-4-RR (same clone as 84215-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SNX18 Recombinant antibody (84215-4-RR)

    WB analysis of mouse brain using 84215-4-RR (same clone as 84215-4-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNX18 Recombinant antibody (84215-4-RR)

    IF Staining of MCF-7 using 84215-4-RR (same clone as 84215-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-4-RR, Clone: 241537D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

Product Information

The immunogen of 84215-4-PBS is SNX18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNX18 fusion protein Ag16645
Full Name sorting nexin 18
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).