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CoraLite® Plus 488-conjugated SNX15 Monoclonal antibody

SNX15 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A10B7

Cat no : CL488-68239

Synonyms

HSAF001435, SNX15, sorting nexin 15

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated SNX15 Monoclonal ant (CL488-68239)

    IF Staining of U2OS using CL488-68239

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SNX15 antibody (CL488-68239, Clone: 1A10B7 ) at dilution of 1:100, CL594-phalloidin (red).

Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68239 targets SNX15 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SNX15 fusion protein Ag8839
Full Name sorting nexin 15
Calculated Molecular Weight 342 aa, 38 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC009897
Gene Symbol SNX15
Gene ID (NCBI) 29907
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SNX15 antibody CL488-68239Download protocol
Standard Protocols
Click here to view our Standard Protocols