SNAP25 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SNAP25 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14903-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis using 14903-1-AP (same clone as 14903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 14903-1-AP (same clone as 14903-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

  IP result of anti-SNAP25 (IP:14903-1-AP, 3ug; Detection:14903-1-AP 1:3500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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Product Information

14903-1-PBS targets SNAP25 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAP25 fusion protein Ag6695
• Full Name: synaptosomal-associated protein, 25kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC010647
• Gene Symbol: SNAP25
• Gene ID (NCBI): 6616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.