SNAI1 Recombinant antibody

SNAI1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230035C9

Cat no : 82214-2-RR

Synonyms

dJ710H13.1, Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

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Validation Data Gallery

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  WB analysis of HeLa using 82214-2-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 82214-2-RR (SNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82214-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82214-2-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82214-2-RR targets SNAI1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNAI1 fusion protein Ag3723
• Full Name: snail homolog 1 (Drosophila)
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC012910
• Gene Symbol: SNAI1
• Gene ID (NCBI): 6615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.