SNAI1 Recombinant antibody, PBS Only

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  WB analysis of HeLa using 82214-2-RR (same clone as 82214-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 82214-2-RR (SNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82214-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82214-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 82214-2-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

SNAI1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 82214-2-PBS

Clone No.230035C9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

dJ710H13.1, Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

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Product Information

82214-2-PBS targets SNAI1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNAI1 fusion protein Ag3723
• Full Name: snail homolog 1 (Drosophila)
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC012910
• Gene Symbol: SNAI1
• Gene ID (NCBI): 6615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95863
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.