SMPD1,ASM Polyclonal antibody

SMPD1,ASM Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30197-1-AP

Synonyms

SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM

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Validation Data Gallery

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  WB analysis of human placenta using 30197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30197-1-AP (SMPD1,ASM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 30197-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SMPD1,ASM antibody (30197-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30197-1-AP targets SMPD1,ASM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMPD1,ASM fusion protein Ag32479
• Full Name: sphingomyelin phosphodiesterase 1, acid lysosomal
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC041164
• Gene Symbol: SMPD1
• Gene ID (NCBI): 6609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing. As a catalytically inactive 75kDa precursor, after signal peptide cleavage, it was processed into smaller non-glycosylated and rapidly degraded 57kDa form in ER-Golgi complex. And 70kDa mature form with major catalytic activity located in lysosomes, which is further processed into 52kDa form (PMID:25853898, PMID:26499107).