SMN (Human,Mouse,Rat) Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SMN (Human,Mouse,Rat) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C10B5

Cat no : 60154-2-PBS

Synonyms

SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541

---

Validation Data Gallery

• 

  WB analysis of HeLa using 60154-2-Ig (same clone as 60154-2-PBS)

  WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 60154-2-Ig (same clone as 60154-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 60154-2-Ig (same clone as 60154-2-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60154-2-Ig (same clone as 60154-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 60154-2-Ig (same clone as 60154-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig, Clone:3C10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

---

Product Information

60154-2-PBS targets SMN (Human,Mouse,Rat) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMN (Human,Mouse,Rat) fusion protein Ag14333
• Full Name: survival of motor neuron 2, centromeric
• Calculated Molecular Weight: 282 aa, 30 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000908
• Gene Symbol: SMN
• Gene ID (NCBI): 6607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.