SMMHC Monoclonal antibody, PBS Only

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  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 60222-1-Ig (same clone as 60222-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

SMMHC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 60222-1-PBS

Clone No.5C6D3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MYH11, Myosin-11, Myosin heavy chain 11, Myosin 11, KIAA0866

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Product Information

60222-1-PBS targets SMMHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMMHC fusion protein Ag13397
• Full Name: myosin, heavy chain 11, smooth muscle
• Calculated Molecular Weight: 1938 aa, 224 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC101677
• Gene Symbol: SMMHC/MYH11
• Gene ID (NCBI): 4629
• RRID: AB_11182594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35749
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.