SMC6 Recombinant antibody

SMC6 Uni-rAb^TM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240015F4

Cat no : 83187-3-RR

Synonyms

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6

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Validation Data Gallery

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  WB analysis using 83187-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 83187-3-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 83187-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 83187-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 83187-3-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SMC6 Recombinant antibody (83187-3-RR, Clone:240015F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83187-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83187-3-RR against Human SMC6 were performed. The affinity constant is 0.428 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue, NIH/3T3 cells, HEK-293 cells
• Positive IF-P detected in: mouse testis tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83187-3-RR targets SMC6 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMC6 fusion protein Ag6898
• Full Name: structural maintenance of chromosomes 6
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC039828
• Gene Symbol: SMC6
• Gene ID (NCBI): 79677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).