SMC6 Recombinant antibody
SMC6 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240015F4
Cat no : 83187-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human testis tissue, NIH/3T3 cells, HEK-293 cells |
Positive IF-P detected in | mouse testis tissue |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83187-3-RR targets SMC6 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SMC6 fusion protein Ag6898 |
Full Name | structural maintenance of chromosomes 6 |
Calculated Molecular Weight | 126 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC039828 |
Gene Symbol | SMC6 |
Gene ID (NCBI) | 79677 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SMC6 antibody 83187-3-RR | Download protocol |
IF protocol for SMC6 antibody 83187-3-RR | Download protocol |
FC protocol for SMC6 antibody 83187-3-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |