SMC6 Recombinant antibody

SMC6 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240015F4

Cat no : 83187-3-RR

Synonyms

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, NIH/3T3 cells, HEK-293 cells
Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83187-3-RR targets SMC6 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMC6 fusion protein Ag6898
Full Name structural maintenance of chromosomes 6
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039828
Gene Symbol SMC6
Gene ID (NCBI) 79677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).

Protocols

Product Specific Protocols
WB protocol for SMC6 antibody 83187-3-RRDownload protocol
IF protocol for SMC6 antibody 83187-3-RRDownload protocol
FC protocol for SMC6 antibody 83187-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols