SMC6 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83187-3-RR (same clone as 83187-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 83187-3-RR (same clone as 83187-3-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83187-3-RR (same clone as 83187-3-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SMC6 Recombinant antibody (83187-3-RR, Clone:240015F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00255-2

  Cytometric bead array standard curve of MP00255-2, SMC6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83187-3-PBS. Detection antibody: 83187-1-PBS. Standard: Ag6898. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83187-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83187-3-RR against Human SMC6 were performed. The affinity constant is 0.428 nM.

SMC6 Uni-rAb^TM Recombinant Antibody for WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83187-3-PBS

Clone No.240015F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6

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Product Information

83187-3-PBS targets SMC6 as part of a matched antibody pair:

MP00255-2: 83187-3-PBS capture and 83187-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMC6 fusion protein Ag6898
• Full Name: structural maintenance of chromosomes 6
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC039828
• Gene Symbol: SMC6
• Gene ID (NCBI): 79677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96SB8
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).