SMC2 Recombinant antibody

SMC2 Uni-rAb^TM Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241536G3

Cat no : 84835-1-RR

Synonyms

SMC protein 2, SMC 2, hCAP E, CAPE, CAP E

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Validation Data Gallery

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  IHC staining of mouse brain using 84835-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84835-1-RR (SMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84835-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84835-1-RR (SMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84835-1-RR is SMC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMC2 fusion protein Ag33471
• Full Name: structural maintenance of chromosomes 2
• Calculated Molecular Weight: 136 kDa
• GenBank Accession Number: NM_001042550.1
• Gene Symbol: SMC2
• Gene ID (NCBI): 10592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).