SMC1A Monoclonal antibody, PBS Only

SMC1A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A5D2

Cat no : 66987-1-PBS

Synonyms

SMC 1A, SMC 1 alpha, Sb1.8, KIAA0178, DXS423E

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Validation Data Gallery

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  WB analysis using 66987-1-Ig (same clone as 66987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66987-1-Ig (SMC1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66987-1-Ig (same clone as 66987-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMC1A Monoclonal antibody (66987-1-Ig, Clone:1A5D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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Product Information

66987-1-PBS targets SMC1A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMC1A fusion protein Ag17257
• Full Name: structural maintenance of chromosomes 1A
• Calculated Molecular Weight: 1233 aa, 143 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC112127
• Gene Symbol: SMC1A
• Gene ID (NCBI): 8243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.