SMAD4 Recombinant antibody, PBS Only (Capture)

SMAD4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240026G9

Cat no : 83169-5-PBS

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

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Validation Data Gallery

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  WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00267-4

  Sandwich ELISA standard curve of MP00267-4, Human SMAD4 Monoclonal Matched Antibody Pair - PBS only. 83169-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0299. 83169-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP00267-4

  The mean human SMAD4 concentration was determined to be 9,362.0 pg/mL in HepG2 cell extract based on a 6.0 mg/mL extract load and 4,272.2 pg/mL in HL-60 cell extract based on a 3 mg/mL extract load.

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  Sample test of MP00267-4

  Serum of sixteen individual healthy human donors were measured. The human SMAD4 concentration of detected samples was determined to be 47.3 pg/mL with a range of ND - 148.8 pg/mL.

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  IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83169-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83169-5-RR against Human SMAD4 were performed. The affinity constant is below 1 pM.

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Product Information

83169-5-PBS targets SMAD4 as part of a matched antibody pair:

MP00267-4: 83169-5-PBS capture and 83169-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMAD4 fusion protein Ag0299
• Full Name: SMAD family member 4
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: BC002379
• Gene Symbol: SMAD4
• Gene ID (NCBI): 4089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.