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CoraLite® Plus 488-conjugated SLP76 Polyclonal antibody

SLP76 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12728

Synonyms

LCP2, SLP-76 tyrosine phosphoprotein, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated SLP76 Polyclonal ant (CL488-12728)

    FC experiment of Jurkat using CL488-12728

    1x10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SLP76 (CL488-12728) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12728 targets SLP76 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLP76 fusion protein Ag3432
Full Name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Calculated Molecular Weight 533 aa, 60 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC016618
Gene Symbol SLP76
Gene ID (NCBI) 3937
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SLP76 antibody CL488-12728Download protocol
Standard Protocols
Click here to view our Standard Protocols