CoraLite® Plus 488-conjugated SLC7A11/xCT Recombinant antibody

SLC7A11/xCT Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230227C5

Cat no : CL488-82115-2

Synonyms

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

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Validation Data Gallery

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  IF Staining of HeLa using CL488-82115-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SLC7A11/xCT antibody (CL488-82115-2, Clone: 230227C5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-82115-2

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using CL488-82115-2

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells, A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82115-2 targets SLC7A11/xCT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC7A11/xCT fusion protein Ag25431
• Full Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC012087
• Gene Symbol: SLC7A11
• Gene ID (NCBI): 23657
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.