SLC25A12 Monoclonal antibody, PBS Only (Detector)

SLC25A12 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B10

Cat no : 67467-1-PBS

Synonyms

AGC1, ARALAR, ARALAR1, SLC25A12

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Validation Data Gallery

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  WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67467-1-Ig (same clone as 67467-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 67467-1-Ig (same clone as 67467-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50650-2

  Cytometric bead array standard curve of MP50650-2, SLC25A12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67467-4-PBS. Detection antibody: 67467-1-PBS. Standard:Ag13576. Range: 1.563-100 ng/mL.

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Product Information

67467-1-PBS targets SLC25A12 as part of a matched antibody pair:

MP50650-2: 67467-4-PBS capture and 67467-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC25A12 fusion protein Ag13576
• Full Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Calculated Molecular Weight: 75 kDa, 63 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC016932
• Gene Symbol: SLC25A12
• Gene ID (NCBI): 8604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.