SLC17A9 Recombinant antibody

SLC17A9 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240328H7

Cat no : 83505-1-RR

Synonyms

Voltage-gated purine nucleotide uniporter SLC17A9, VNUT, Vesicular nucleotide transporter, FLJ23412, C20orf59

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Validation Data Gallery

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  WB analysis using 83505-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83505-1-RR (SLC17A9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 83505-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 83505-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83505-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Caco-2 using 83505-1-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC17A9 Recombinant antibody (83505-1-RR, Clone:240328H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83505-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83505-1-RR against Human SLC17A9 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse pancreas tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83505-1-RR is SLC17A9 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC17A9 fusion protein Ag24970
• Full Name: solute carrier family 17, member 9
• Calculated Molecular Weight: 436 aa, 47 kDa
• Observed Molecular Weight: 50 kD
• GenBank Accession Number: BC025312
• Gene Symbol: SLC17A9
• Gene ID (NCBI): 63910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.