VGLUT2 Recombinant antibody

VGLUT2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241255B5

Cat no : 84083-2-RR

Synonyms

SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, 241255B5

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Validation Data Gallery

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  WB analysis of mouse brain using 84083-2-RR

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84083-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84083-2-RR against Human VGLUT2 were performed. The affinity constant is 1.48 nM.

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Tested Applications

• Positive WB detected in: unboiled mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84083-2-RR is VGLUT2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VGLUT2 fusion protein Ag29565
• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_020346
• Gene Symbol: VGLUT2
• Gene ID (NCBI): 57084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).